The integration of Internet of Things (IoT) technologies with biotechnology is revolutionizing modern agriculture by enabling data-driven, sustainable, and precise farming practices. This chapter highlights how IoT devices, such as sensors and wireless networks, collect real-time data on soil health, crop conditions, and environmental factors, providing a robust foundation for biotechnology applications. Concurrently, advances in biotechnology ranging from genetic modification and biofertilizers to engineered microbes enhance crop resilience, productivity, and disease resistance. When combined, these technologies enable precision agriculture that minimizes resource waste, reduces chemical input, and improves overall farm efficiency. The chapter further explores innovations such as biosensors for microbial monitoring, machine learning for disease prediction, and the use of microbial consortia tailored to IoT-informed conditions. These synergies contribute not only to higher yields and sustainable resource management but also to broader goals like climate resilience and food security. Additionally, the convergence of IoT and biotechnology is expanding into environmental monitoring and personalized health applications. By bridging real-time sensing with biological innovation, this integrated approach supports a transition toward intelligent, eco-friendly agricultural systems that can adapt to the evolving demands of a growing global population and a changing climate.