Analysis of Reactive Oxygen/Nitrogen Species in Cold Atmospheric Plasma Activated Water

Cold atmospheric plasma (CAP) is generated by excitation of plasma sources with different structures at atmospheric pressure. It has good tolerance to tissues and cells, has shown great benefit and potential in promoting wound healing, resisting bacterial and fungal infections, inducing cell proliferation or apoptosis, and inducing stem cell differentiation. At the micro level, CAP is a non-bound system comprising various free radicals, charged particles, ultraviolet radiation, and excited particles, which is characterized by high safety and strong ionic activity. Free radicals such as reactive oxygen species and reactive nitrogen are the main effector molecules of CAP. In this study, CAP was used to treat deionized water to obtain plasma activated water. The concentrations of several key active oxygen and nitrogen ions in activated water were measured, as well as the variation of ion concentration with the treatment time of the device. The research results can provide valuable references for the research and application of plasma medicine in vivo and in vitro.