Insight into 3D Printed Eight Well Electrochemiluminescence Biosensing Platforms with Shared Cathode: Towards Multiplexed Sensing

Additive manufacturing processes have shown the potential to fulfil the growing demand for developing low-cost, miniaturized biosensing platforms with high precision and accuracy. In this work, a 3D printed cathode shared closed bipolar electrode eight-well electrochemiluminescence platform is developed for performing multiplexed sensing of various analytes with different concentration ranges simultaneously. The most common luminophore derivatives, ruthenium and luminol, for cellular analysis were explored for multiplex sensing in the same platform. In Luminol/H2O2-based sensing, the varying concentration of H2O2 has shown a linear range from 0.1 to 10 mM with a limit of detection (LOD: 0.1 mM). Similarly, in Ru(bpy)32+/TPrA sensing, the varying concentration of Ru(bpy)32+ has shown a linear range from 100 to 700 µM with a limit of detection (LOD: 30 µM). The proposed device may perform multiplex sensing based on ruthenium and luminol for biological samples.