This chapter examines the gene bank procedures that influence the status of conservation of Colombian plant agrobiodiversity, focusing on key areas of the functions of both active and base collections, highlighting the importance of linking plant conservation with its real use. This strategy encompasses acquiring, characterizing, documenting, and utilizing genetic resources. Germplasm banks strategically distributed across the country maintain a substantial stock to conserve and study plant, animal, and microorganism diversity for efficient integration into production programs. The Plant Germplasm Bank (BGV), a key player in this endeavor, employs three conservation systems. Seed collection included taxa with orthodox seeds, recalcitrant field-conserved species, and in vitro-conserved species. Novel characterization technologies play a critical role in detecting and associating traits of interest and in expediting breeding processes. Systematizing information for each collection is a priority, and efforts have been made to ensure traceability and to implement and update the GRIN-Global platform. Dissemination strategies have been developed to promote the use of the BGV, with an emphasis on research, technological development, and innovation. These efforts showcase the conservation of richness and diversity and encourage their utilization through strategic initiatives. Incorporating technologies and various dissemination mechanisms collectively enhances awareness and accessibility, fostering responsible use and innovation in the realm of genetic resource management in Colombia. This chapter describes the different conservation strategies for Colombia’s agrobiodiversity developed by the BGV in response to the threats posed by transformative factors responsible for reducing genetic diversity in species that are important for food and agriculture.