Continuous welded rail (CWR), considerable longitudinal forces may develop due to temperature variation, train loadings like braking, traction, and deck end rotation due to vertical bending. Rail structure interaction (RSI) analysis is an integral part of bridge design to evaluate the longitudinal stresses in CWR, longitudinal forces on the substructure, and deflection/displacement of the structure. The stress developed in CWR significantly depends on the girder and substructure's stiffness. In this present study, RSI is done by using Midas Civil software. RSI analysis performed by two methods are Simplified Separate Analysis and Complete Staged Analysis. Here, considering three cases for analysis are 3 Span of 20 m, 3 Span of 30 m and 3 Span of 40 m bridge length. Results have been checked as per Union Internationale des Chemins de fer internationaler Eisenbahnerband International Union of Railways (UIC 774-3R) and Indian Railway Standards Bridge Rules (IRS Bridge Rules) codes. It is observe that the most favorable bed is a concrete bed that satisfies all the limits as per the codes given on Prestressed Concrete PSC Box Girder, and a complete staged analysis gives the exact result.