On a global scale, climate change and population explosion pose a substantial risk to food security and nutritional quality. Genome engineering through a novel CRISPR/Cas technique is one of the most promising transformative tools for addressing issues related to crop productivity, safety, and biofortification challenges and fulfills the demands of an ever-increasing population. This technology enables precise and efficient modifications of plant genomes, allowing researchers to enhance traits related to nutrition, such as increasing the content of essential vitamins, minerals, and other bioactive compounds. CRISPR/Cas-based knock-in and knockout approaches have been successfully employed to improve crop yield and fortify crops with key nutrients, contributing to the production of safer and more nutritious food. Moreover, reports on biotic and abiotic stress management in important food crops through CRISPR/Cas approach validate their efficiency and potential as a tool for producing stress-resilient plants. This chapter explores the current advances and future potential of CRISPR/Cas technology in crop improvement, emphasizing its critical role in producing nutritious, safe, and sustainable food for a growing global population.