Submerged flow conditions are often encountered in coastal environments, offshore structures, bridge piers, and caisson foundations. The understanding of hydrodynamics between two adjacent submerged circular cylinders is important in its design and problems like scouring with these structures. The spacing between two submerged circular cylinders is important in flow parameters such as flow field, mean velocities, Reynold’s stress, turbulence intensities, and streamline patterns around. In this study, the hydrodynamics around two submerged circular cylinders are analyzed experimentally. A series of experiments were performed in a recirculated rectangular glass open channel having dimensions of 15 m × 0.50 m × 0.90 m in length, width, and height respectively. Two circular cylinders of diameter (d) 0.03 m were mounted on a plane rigid bed along the channel center and located approximately 10.3 m away from the channel inlet. The experiments are conducted with four face to face spacing (L) conditions, i.e., L = 0d, 2d, and 3d. The submergence ratio (S), which is the ratio of water depth above the cylinder to the total flow depth (H), is taken as 0.52. The entire flow field of instantaneous velocity vectors were collected along the plane of symmetry using an advanced Particle Image Velocimetry (PIV) system. The changes in the velocity fields, vortex generation, expansion, and concentration are observed in this study. The acceleration in the velocity magnitudes, flow separation at the cylinder top surface and downwash flow at the tip of the cylinders are described in detail. The upstream region is not greatly affected in all spacing conditions due to the same blockage area. The concentration of the negative velocity region shifts towards the bed region as spacing increases. It is evident from the calculations that the bed shear stress reduced away from the rear end of the cylinder and the higher magnitude values are observed at the immediate downstream end of the front cylinder. The effects of spacing effect on various parameters are analyzed and the experimental findings are presented. The results from the study will be useful in the design of piers under submerged conditions.