Plants have historically served as invaluable reservoirs of essential compounds utilized across various industries, including food, healthcare, and agrochemicals. Due to increased global demand in pharma and nutraceutical sectors, there is overexploitation leading to rampant habitat loss, exacerbated by environmental degradation. To address this challenge, alternative technologies for the commercial production of phytochemicals are imperative. Traditional cultivation or sourcing from wild are laborious, time-intensive, and often yield limited quantities, particularly for high-value secondary metabolites. In response, in vitro tissue culture has emerged as promising sustainable option. Yet, these systems too face constraints in achieving commercially viable yields. Elicitors, mimicking environmental stress factors, trigger a cascade of metabolic events as a protective mechanism. By subjecting tissue culture systems to these elicitors, researchers can stimulate the production of targeted compounds, offering a sustainable solution to the challenges of traditional methods. The role of different elicitors in augmenting bioactive phytochemicals within the invitro system is presented here. Further the mechanisms of action of elicitors, their application in enhancing metabolite content, and the potential for scaling up production for industrial use is discussed. This chapter provides insights into the utilization of elicitors as a key strategy in the quest for sustainable and efficient production of bioactive compounds essential for various sectors, including food and healthcare.