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Practical protocols for genetic engineering to enhance secondary metabolite production in plants includes detailed methodologies for genome editing using CRISPR/Cas9 and plant transformation techniques involving Agrobacterium tumefaciens. Specific procedures cover the manipulation of the Pun1 gene in Capsicum annuum and the introduction of target genes into tobacco plants. The protocols aim to equip researchers with skills for performing genetic modifications, screening for successful transformations, and analyzing resulting phenotypic changes. These methods advance research in plant biotechnology and metabolic engineering by enabling effective manipulation of plant genomes.

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Practical Approaches for Metabolic Engineering

  • 章节

  • 发表日期：

    2025年

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  • Sudipta Kumar Mohanty,
  • B. K. Indu,
  • Sharad S. Achar,
  • G. Suhasa

摘要

Practical protocols for genetic engineering to enhance secondary metabolite production in plants includes detailed methodologies for genome editing using CRISPR/Cas9 and plant transformation techniques involving Agrobacterium tumefaciens. Specific procedures cover the manipulation of the Pun1 gene in Capsicum annuum and the introduction of target genes into tobacco plants. The protocols aim to equip researchers with skills for performing genetic modifications, screening for successful transformations, and analyzing resulting phenotypic changes. These methods advance research in plant biotechnology and metabolic engineering by enabling effective manipulation of plant genomes.