Soybean plants exhibit a high water demand and exhibit wilting and drooping of leaves when water stress occurs. As a response to water stress, a complex network of events pertaining to molecular physiological and molecular alterations are modulated, in accordance to the intensity of water deficit they come across. One of the acclimated responses to these stress conditions is the osmotic adjustment and stomatal closure mediated through abscisic acid. The osmotic changes are mediated through accumulation of several molecules including sugars, sugar alcohols, amino acids, and other osmoprotectants. The response to water deficit is mostly relied on the expression and regulation of thousands of genes The complex signaling cascade involves the transcription factor expression that is responsible in downregulating the downstream genes such as RD20, ERD1, RD29 B, and RD20 A, the commonly used water-deficit marker genes. The expression levels of Gmax ERD1-like was four times higher in sensitive cultivar of soybean, which suggests that sensitive cultivars are more sensitive to water stress at the molecular level, due to a lack of traits in them that confer adaptation to drought. In sensitive and tolerant cultivars of soybean, GmaxRD20-like expression was identical. An exhaustive search of soybean genome has shown that Glyma16g31330 was a similar gene to that of Arabidopsis RD29 B. In the promoter regions of differentially methylated genes there was a high frequency of cis elements ACGT, ACCGAC, WAACCA, and CANNTG, which has suggested that these elements might be of importance in the expression activity of genes during water deficit.