This chapter discusses the application of BoCs technology in studying BC and outlines the challenges, limitations, and future directions in this domain. This emphasizes the limitations of traditional research methodologies in accurately mimicking the complex dynamics of BC and highlights the emergence of OoC technology as a revolutionary tool for replicating the physiological and mechanical functions of human organs, including the breast. The integration of natural compounds with BoC technology, particularly Acacia gums (AGs), has been explored as a novel approach in BC research, offering potential therapeutic avenues for disrupting the metastatic cascade and enriching our understanding of BC pathophysiology. The challenges and limitations in applying BoC technology to study BC are discussed, including the technical complexity of accurately mimicking the tumor microenvironment (TME), maintaining cell viability, and integrating immune system components. However, the future of BoC technology appears promising, with potential advancements in biomaterials, microfluidic design, three-dimensional (3D) printing, nanofabrication, and the integration of immune system components and stem cell technology. These advancements could lead to improved replication of tumor complexity, personalized medicine, high-throughput drug screening, and transformative impacts on BC research, paving the way for groundbreaking discoveries and advancements in treatment methodologies. In conclusion, this chapter underscores the immense potential of BoCs technology in advancing our understanding of BC and improving treatment strategies by providing a more accurate and sophisticated platform for studying the complex architecture and cellular interactions of the breast TME.