The plant virus species of the family Benyviridae (order Hepelivirales) have multipartite plant viruses with rod-shaped virions, with the species beet necrotic yellow vein virus (BNYVV) being the type benyvirus (Gilmer and Ratti 2017; Koonin et al. 2020). Members of this virus family are transmitted by soilborne plant endoparasites, plasmodiophorid protists of the genus Polymyxa (Adl et al. 2012). These are single-stranded, positive-sense RNA genome with four segments containing a 5′-terminal “cap” structure and a 3′ poly(A) tract, and there is post-translational cleavage of the viral replicase (Gilmer and Ratti 2017). However, RNAs 1 and 2 may cause productive infection. RNA1 encodes a replicative protein with several typical domains, whereas RNA2 includes the 5′-terminal capsid protein (CP) ORF1 extended by a read-through ORF2, a triple gene block (TGB) movement module, and a cysteine-rich protein-encoding ORF6 (Pakdel et al. 2015; Gilmer and Ratti 2017; Niehl et al. 2021). The family contains a single genus, Benyvirus (Saito et al. 1996; Gilmer and Ratti 2017; Niehl et al. 2021). Benyviruses can persist in the soil for decades through resting spores dormant on the vector (Abe and Tamada 1986). The genus Benyvirus comprises four species, which include beet necrotic yellow vein virus (BNYVV, the type member), burdock mottle virus (BdMV), beet soil-borne mosaic virus (BSBMV), rice stripe necrosis virus (RSNV) members, and mangifera indica latent virus (MILV) a tentative member (Sela et al. 2016) [New Taxonomy Release: MSL38—2022 v3. https://ictv.global/news/taxonomy-2022.3 ]. The general characteristics of this family can be found in Table 22.1.