Vegetative propagation aids tree improvement programme. Over a period of time the technique has been developed for many species, yet, there is ample scope for research especially addressing maturity-related decine in rooting, physiology, genetics and molecular biology of adventitious root formation in woody perennials. This will enable development of rapid, efficient and cost-effective methods of clonal propagation for tree species of commercial importance. Not only induction of appropriate quantity of adventitious roots, but the quality of the parent material and the produced plantlets are equally important in clonal propagation. The root architecture in case of clonally propagated trees plays an important role in survival and growth of the tree. Inter- and intra-specific variability in adventitious rhizogenesis needs to be studied to generate information which may be of great value for evolving uniform and workable clonal protocols. Eucalyptus can serve as an ideal system for such studies as evident from recent research. Eucalyptus clonal propagation is practised extensively and it serves as a good example for understanding the concept of juvenility, difference between leafy and non-leafy cuttings, hormonal treatments required to induce rooting, environmental requirement, etc. This chapter discusses the technique of propagation of Eucalyptus species through leafy stem cutting collected from the juvenile coppice shoots. Mist chamber propagation is a technique that uses high humidity and temperatures to promote adventitious root growth in leafy stem cuttings. The cuttings are hardened under shade house conditions with irrigation and nutrition control. Various rooting techniques and variables are described.