Biodiesel is a low-carbon alternative energy source that can be used as a substitute for fossil fuels. Transesterification of oil and alcohol under mild reaction conditions is carried out assisted by high shear mixing to produce biodiesel in a shorter reaction time. The reaction time is reduced due to the formation of a fine emulsion between oil and alcohol, and the yield of biodiesel produced is higher than that produced by conventional methods. In addition, the cost of biodiesel production, which is a major barrier to biodiesel commercialization, can be reduced by using low-quality oils such as crude rice bran oil (CRBO). Several parameters affect this process, including acid catalyst concentration, reaction time, dispersion rate, and ethanol: CRBO molar ratio. Biodiesel production increased significantly when the dispersion rate was increased to 8000 rpm and the reaction time to 8 min. At 1% w/w acid concentration, ethanol: CRBO 1:12, 10,000 rpm, and 10 min reaction duration, the highest biodiesel yield of 74.58% was achieved. Optimization of biodiesel production using Historical Data Design (HDD) and Response Surface Methodology (RSM), with biodiesel yield as the response. Optimization resulted in a catalyst concentration of 3.3%, reaction time of 5.6 min, ethanol: Ethanol molar ratio: CRBO molar ratio of 27.8:1, and dispersion speed of 4747 rpm.