Acoustic studies in biological media have emerged as a dynamic and interdisciplinary field of research, offering profound insights into the intricate relationships between sound and living organisms. This chapter provides a concise overview of the multifaceted investigations within this burgeoning domain, shedding light on the fundamental principles, cutting-edge methodologies, and promising applications that define its significance. It covers the interaction of acoustic waves with biological tissues, imaging modalities, and emerging advancements in the field. In the realm of biological media, the use of acoustic waves as a probing tool has uncovered a multitude of revelations. These studies delve into the diverse mechanisms by which organisms generate, perceive, and interact with sound. Moreover, the field of acoustic studies in biological media leverages advanced techniques such as ultrasound imaging, sonar systems, and underwater acoustics to explore the anatomical and physiological aspects of living organisms. This has profound implications for medical diagnostics, non-invasive monitoring of health, and the preservation of endangered species, among other applications. Biological media, whether in the form of biological tissues, aquatic environments, or terrestrial ecosystems, present unique challenges and opportunities for acoustic exploration. Researchers employ a variety of tools, ranging from state-of-the-art hydrophones and transducers to computational models and artificial intelligence algorithms, to unravel the secrets concealed within these complex acoustic landscapes. In a nut shell, acoustic studies in biological media offer a captivating journey into the sonic world of nature, where scientists unravel the mysteries of communication, navigation, and adaptation in living organisms. The ever-evolving methodologies and applications in this field hold great promise for enhancing our understanding of the natural world, improving healthcare, and advancing environmental conservation efforts. As the frontier of acoustic research in biology continues to expand, its potential to transform our knowledge of life on Earth remains boundless.