The acoustic properties of the material are defined by the reflection coefficient, absorption coefficient, and transmission coefficient. The experimental setup follows the ASTM-2611 standard of four-microphone impedance tubes with two load boundary conditions to measure these coefficients. The setup consists of four microphones around a brass tube with the speaker at one end and termination at the other. Raw data from the four microphones is obtained through a Virtual Instrument (VI) program developed in LabView. The mathematical equations involved in estimating acoustical properties is solved in MATLAB 2019a. The reflection and absorption coefficient data of ephony fibbrette of 15 mm thickness are compared with the data provided by an accredited laboratory. This material is used in the auditorium, and theaters, for hearing comfort. Further, two new samples of ephony fibbrette along with wood fiber cement and damper have been analyzed.