This study represents an innovative approach of increasing cryptographic security by using physiological data, marking a significant stride towards safeguarding digital information across diverse fields. The pulse and temperature information are utilized to create and optimize the keys through a multiple linear regression model. Implementing a triple-layer encryption architecture with One Time Pad (OTP), DNA encoding, and AES algorithm shows a robust level of security in the digital communication channel. This paper contributes to the development of cryptographic security, enhances efficiency and security, and the results show that the suggested cryptosystem has an exceptional capacity to resist known attacks.