Silk fibroin is a versatile polymer that occurs naturally and is of great importance in various scientific domains owing to its outstanding properties. It is quite difficult to achieve proper fiber-matrix adhesion because of the hydrophilic sericin layer on the fiber surface. Thus, degumming the fiber surface enables greater control over the fiber’s characteristics. Several degumming techniques are available for extracting the silk fibroins. This paper focuses on the various degumming methods, including acid, alkali, and enzyme degumming. The degumming loss associated with these methods is 25.4%, 24.32%, and 22.73%, respectively. Recently, green alternative degumming treatments were discovered, such as microwave irradiation, ultrasonication, and autoclaving, with degumming loss of 25.5% and 16.33%, respectively. The impact of degumming on the mechanical properties, structure, molecular weight, and morphology are discussed in this chapter. An emphasis has been put on the various degumming treatments for silk fibroin extraction with a discussion of the in-depth knowledge of degumming influence on the properties of silk fibers along with the applications of silk fibroin in detail.