The commonly object used as a review regarding of biofouling invasion in previous research was ship made of steel. Therefore, in this study a 1 GT wooden fishing vessel with 8.00 m in length, 2.00 m in width, and 0.90 m in height is used to obtain its performance during the invasion of biofouling, include the ship stability, ship resistance, and the growth of the macro fouling. The methods used is experimental methods and numerical simulation using Maxsurf. In the first three, four, and six months, the average adhered macro fouling thickness under the draft is 3, 5 and 7 mm successively. The most growth of biofouling occurred on the planking joint and on the rudder compared to other area of hull. However, in a square of 10 × 10 cm, there were around 52 barnacles in the first three month after the ship was launched. This continued to grow approximately 33 barnacles after a month later. Finally, in the sixth month, total barnacles on the hull reached roughly 110 barnacles. Meanwhile, total resistance of the vessel before the invasion of biofouling was 3.1 kN, then it increased to 3.2 kN after 3 months during the invasion. In the following month, it gradually went up to 3.7 kN, and roughly 6.7 kN in the end of sixth month. The stability criterion of the wooden fishing vessels before and after the invasion meet the requirement of IMO.