This study focuses on the effect of a hot and cold environment on the sensation of the human body. The subjective analysis consists of a set of questions that gauge the thermal state of a person in terms of thermal sensation votes, etc. Experimentally, the hot and cold environments are simulated in a climate chamber. For the hot environments, experiments are performed at 30, 33, 36 and 39 °C, and for the cold environments, experiments are performed at 6, 9, 12 and 15 °C. The study is performed for the metabolic rate of 2 Met. This metabolic rate is obtained by making the subjects walking at a speed of 3.2 km/h on a treadmill. The subjective response is taken before and after walking for all the experiments. The linear regression equations are fitted between thermal sensation votes (TSV) with ambient temperature for all the experimental conditions. For finding neutral temperature, the value of TSV is equated to zero, which denotes a thermally comfortable environment. The variations of neutral temperature are investigated for different metabolic rates.