Growing interest in the use of nanotechnology in agriculture for the purpose of enhancing plant growth and production has been seen in recent years. The unique qualities of silver nanoparticles (AgNPs), such as their capacity to improve nutrient absorption and antibacterial activity, have made them stand out as potential candidates. Different variables, such as soil characteristics, exposure time, concentration, and size of the nanoparticles, affect these interactions. AgNPs at low concentrations stimulate the growth of arbuscular mycorrhizal fungus (AMF), maybe through modifications to the soil microbial habitat. On the other hand, increased AgNP concentrations restrict the growth of AMF, which is linked to their cytotoxic effects and disruption of symbiotic relationships. Simultaneously, AMF and plants have long been known to share a symbiotic relationship that promotes nutrient uptake and stress tolerance. AMF colonization affects AgNP uptake, distribution, and toxicity in plants, emphasizing the function of mycorrhizal symbiosis in reducing stress brought on by AgNP. This chapter highlights the effect of arbuscular mycorrhizal fungi and silver nanoparticles as well as the impact of AgNPs and AMF interaction on plant development and productivity within a soil matrix.