The emergence of digital twin technology has facilitated the assessment of the level of water ecological civilization in the Yangtze River basin. In recent years, the digital twin technology in the Yangtze River basin has led to a gradual improvement in the efficiency of water quality and environment monitoring. In this context, how to flexibly apply digital twin technology to measure the development level of ecological civilization in the Yangtze River basin? In order to solve this problem, this paper firstly builds the evaluation index system of water ecological civilization level in Yangtze River basin based on DPSIRM model, and introduces Entropy weighting technique. Then analyzes the impact of digital twin technology on each index dimension, and finally designs how digital twin construction can better promote the construction of water ecological civilization in Yangtze River basin.