Computational Analysis of the Putative Endoglucanases in the Genome of Cellulomonas flavigena ATCC 53703

Cellulomonas flavigena is a Gram-positive bacteria that produces cellulases and xylanases, which may break down cellulose, hemicellulose, and xylan. In this work, we used many in silico technologies to investigate the whole genome of Cellulomonas flavigena. The endoglucanases were predicted to belong to the glycosyl hydrolase family using BLAST and Conserved Domain analysis. The endoglucanases were discovered to be stable, as expected by the pI and aliphatic index. Homology modeling yielded high-quality structural models of Cellulomonas flavigena endoglucanases protein. Cellulomonas flavigena endoglucanases proteins were docked with cellulose. Protein–protein interactions were predicted using STRING databases. According to a KEGG pathway study, endoglucanase in Cellulomonas flavigena plays an important function in cellulose metabolism. The results of the in silico study agreed with those of the enzyme test and kinetic analysis.