The current study presents the effect of slip on the linear stability analysis of Newtonian fluids flowing in two layers within an infinitely long 2D channel. This two-layer flow system involves the mass transfer of species from the upper fluid layer to the lower fluid layer, with the upper wall having a more constant concentration of species compared to the lower wall. In this setup, the lower wall of the channel is treated as a slippery surface. The analysis focuses on the effect of slip on the Marangoni instability by implementing a slip condition on the lower wall of the channel. The results reveal that the application of slip to the lower wall of the channel can effectively regulate the stability of the two-layered flow system in the presence of soluble species.