Stability has a dominant role over preserving the thermophysical properties of nanofluids for longer durations from their development. Rapid decrement in the balance between repulsive and attractive forces of nanoparticles can promote the agglomeration in the suspension and deteriorate the thermal performance with the drastic loss of colloidal characteristics. Various techniques such as magnetic stirring, ultrasonication, surfactants, and pH modifiers are applied to improve the chemical stability of nanofluids. But the stability improvement techniques can have significant influence over the thermal conductivity of nanofluids with the effect of intermolecular reactions. The study is aimed to investigate the influence of stability enhancement techniques over the thermophysical properties such as thermal conductivity, viscosity, and density. The nanofluid at different volume fractions such as 0.03, 0.06, 0.09, and 0.12% are developed by dispersing 35 nm sized Al2O3 nanoparticles into deionized (DI) water, and stability improvement techniques are applied. The observations made from the performed experimentation are discussed with suitable illustrations.