In an effort to promote wound healing, rapid hemostasis is accomplished through lowering bleeding and minimizing infection. In particular, calcium plays a significant role in contact activation of hemorrhage conditions of injured blood vessels, which accelerates the blood clotting pathways. This work presents the preparation and characterization of an affordable hemostatic film by using Oyster shell (OS) derived green marine waste powder, Polyvinyl alcohol (PVA), and Chitosan (CS) by an air-dried casting method. The results obtained from the Raman spectroscopy, contact angle, and UTM confirmed the conjugation of PVA, Chitosan, and OS powder with enhanced hydrophilicity and improved tensile strength. The developed films showed significant results in CT, PT, APTT, PRT, swelling, and degradation analysis. Hemolysis study showed the film materials are absolutely hamocompatible and also have antimicrobial properties as noted from the disk diffusion method.