Urbanization leads to increased volume of stormwater and peak flow, resulting in flood disasters. Rain garden is a reliable best management practice. Rain garden reduces and delays flood peaks, helps groundwater recharge, and enhances biodiversity. Additionally, its vegetation works as a filter media for stormwater treatment. Unfortunately, due to little past knowledge and design guidelines, significant difficulties continue to exist in the construction and design of these systems. The present study aims to study the infiltration characteristics of rain gardens with different types of vegetation and flow conditions. Four rain gardens were constructed in the Hydraulics Laboratory of NIT, Kurukshetra, India. Vegetation used during the study is scutch grass (cynodon dactylon), Chandni flower (candytuft), marigold, and daisy flower plants. Results indicate that the average infiltration rate is minimum for bare rain gardens. The garden with a higher number of scutch grass plants infiltrates more quickly than daisy and candytuft plants. With an increase in the height of vegetation, the infiltration rate is improved. The infiltration rate of rain gardens under overflow situations is higher.