The value of textiles in maintaining a comfortable internal body temperature cannot be overstated. Through metabolic processes, the human body generates heat constantly at a rate between 40 and 440 W/m2 depending on the nature of their activities. The thermal insulation that is provided to the person wearing the severe cold weather apparel ensemble comes from the employment of high-bulk nonwovens that are arranged in the form of layers. In this research, two different multi-layered fabrics have been studied with the same inner and outer layers but varying middle layers. Polyester knitted fabric is used as the inner layer, and PU-coated nylon fabric is used as the outer layer. Two different types of hollow fibers are used for the middle layer in the form of waddings. The hollowness percentage of these fibers is calculated by using Scanning Electron Microscope (SEM) analysis at different magnification levels and found that the hollow polyester (3.33 Tex) fiber shows a higher hollowness percentage than Thermolite® fiber (0.15 Tex). Thermal properties such as thermal resistance, thermal conductivity, thermal diffusivity, thermal absorptivity, and value of heat flux have been evaluated for both fabrics using the Alambeta instrument. Along with that the effect of areal density (GSM), porosity, and thickness of the middle layer on multi-layered fabric structure with respect to thermal properties also have been studied in this research. The results of both fabrics are analyzed using ANOVA and it is concluded that multi-layered fabric with Thermolite® fibers waddings as the middle layer shows lower thermal conductivity, thermal absorptivity, and higher thermal resistance and thermal diffusivity with respect to the fabric with hollow polyester fibers waddings.