Clothing is one of the fundamental requirements of human beings which gives the required comfort and protects from extreme climates. There are numerous interactions between fabric and human skin that influence comfort level. The nature of the fabric, its blend and thermal and moisture management behaviour largely affect the comfort level of the fabric. Woven fabrics are one of the essential clothing components in our everyday life. In recent times large volumes of plastic waste is generated which brings with it a colossal challenge of waste management. Refurbishing plastic waste into value-added textiles is one of the potent options for handling waste as well as resource conservation. There is a need to design fabrics from recycled materials. This paper focuses on the impact of incorporating recycled Polyester fibre in woven fabric. This research aims to explore the effect of yarn blend, linear density and twist multiplier on the thermophysiological properties of woven fabrics made from Recycled Polyester wool blends. The assessment of the thermal comfort of fabrics was done on the basis of instrumental parameters such as air permeability, water vapour permeability, thermal conductivity, thermal resistance, thermal absorptivity, etc. It was observed that with increasing yarn fineness and twist level the air permeability, as well as water vapour permeability, were found to increase whereas thermal comfort properties showed a close association with wool content.