Analyzing of 6-APA for Synthesis of Amoxicillin

6-Aminopenicillanic acid is an important intermediate to produce antibiotics in pharmaceutical industry all over the world. There are many sources to produce 6-APA (6-Aminopenicillanic acid), but still natural penicillin is the major source. This originated from penicillin and cephalosporin with the help of penicillin and cephalosporin C amidase. The commonly used raw material is Penicillin G (PEN-G) for semi-synthetic antibiotics. The present study is based on the impurities shown in 6-Aminopenicillanic acid by HPLC and IR methods. The high number of impurities can also have a huge impact on the pharmaceutical industry by lowering the shelf life of related substances, causing problems in formulations as well as health issues. Furthermore, fermentation is performed in the synthesis of raw material of 6-APA (6-Aminopenicillanic acid) with the help of an enzyme. PEN -G (Penicillin G) can further be converted either chemically or enzymatically to APA (Aminopenicillanic acid) and PAA (phenyl acetic acid). The testing of 6-APA (6-Aminopenicillanic acid) in the HPLC (High performance liquid chromatography) method experimentally revealed two peaks of PEN-G (Penicillin G) and PAA (phenyl acetic acid) are generated where their specifications should not more than 0.50% w/w to achieve the safety measure of a drug in pharmaceutical industry and hence can further used for the synthesis of antibiotics such as amoxicillin.