Hospital ward layout is greatly influenced by energy performance and daylighting studies, which also has an impact on staff well-being, patient outcomes, operational efficiency, regulatory compliance, and environmental stewardship. This study delves into the intricate relationship between space layout design variables and their effects on both daylight provision and energy performance of government hospital general wards situated in warm-humid climates of India. The investigation employs a comprehensive methodology encompassing simulation-based analysis using the EnergyPlus simulation engine and DesignBuilder software to evaluate the various design parameters of a calibrated simulation model of the reference hospital. Key variables such as orientation, window configurations as well as shading devices are systematically examined to determine their individual and collective impacts. Findings from this research not only clarify the complex relationship between space layout design variables and energy performance but also offer actionable insights for architects, designers, and healthcare practitioners. By identifying optimal design solutions that balance daylighting requirements with energy efficiency goals, this study endeavors to enhance the quality of healthcare environments while promoting sustainable building practices tailored to the specific climatic challenges of warm-humid regions. Ultimately, the outcomes of this study contribute to the advancement of evidence-based design principles for hospital wards, fostering environments that prioritize patient comfort, staff well-being, and operational sustainability.