Engineering of Persistent Luminescence Nanoparticles for Biosensing

Because of their unique optical features, persistent luminescence nanoparticles (PLNPs) have emerged as a viable tool for biosensing applications. After being activated by an external light source, these nanoparticles may emit light for a sustained period of time, making them ideal for long-term imaging and detection in biological systems. The PLNLs have attracted considerable attention for biosensing due to their unique advantages such as high signal-to-noise ratio, low background interference, and multiplexing capability. To engineer PLNPs for biosensing, several key factors need to be considered, including the choice of host material, dopant ions, and surface modifications. The design and development of nanoparticles with persistent luminescence for use in biosensing applications are covered in this chapter. This includes a discussion of different types of persistent luminescence nanoparticles, their synthesis and surface modification, and the key parameters that affect their performance as biosensors. The chapter also highlights the various applications of persistent luminescence nanoparticles in biosensing, including in vitro and in vivo sensing, and discusses the advantages and limitations of this technology.