Methodology to Study Tryptophan Metabolism
摘要
This chapter introduces the current approaches to extract, separate, and detect tryptophan and its metabolites. Furthermore, high-throughput omics have also been included in this chapter to describe the large-scale study of tryptophan (Trp) metabolism. Among them, the detection of tryptophan and its metabolites can be achieved through high-performance liquid chromatography, capillary electrophoresis, near-infrared spectroscopy, amino acid analyzer, direct or indirect colorimetry, biosensors, or mass spectrometry. In addition, high-throughput metabolomics analysis is commonly used for rapid detection of Trp and its metabolites.