Effects of Glyphosate and Antibiotics on the Expression of Genes Related to Performance, Antioxidant Protection and Histological Barrier in the Cecum of Broilers
摘要
In conditions of intensive poultry farming, significant amounts of xenobiotics enter the bird’s body. To investigate this, four groups of Ross 308 broiler chickens were formed: (1) control group fed the basic diet (BD); (2) experimental group fed BD supplemented with glyphosate; (3) experimental group fed BD along with combination of glyphosate and two antibiotics, enrofloxacin and colistin methanesulfonate. Analysis of the expression of genes for performance (IGF1, IGF2, MYOG, MYOZ2, SLC2A1, SLC2A2, SLC5A1, MSTN and TGFB1), antioxidant defense (CAT, SOD1, PRDX6 and HMOX1) and histological barrier function (MUC2, OCLN and CLDN1) in cecal tissues of birds were carried out using quantitative RT-PCR using a DTlight thermal cycler (DNA-Technology, Russia) and the SsoAdvancedTM Universal SYBR® Green Supermix kit (Bio-Rad, USA). The results showed that glyphosate alone (Group 2) inhibited the expression of a number of genes associated with productivity (IGF1, IGF2, SLC5A1 and MSTN) up to 4.1 times as compared with Group 1 (p < 0.05). In Groups 2 and 3, there was a decrease in almost all cases in the mRNA production of the MUC2, OCLN and CLDN1 genes in intestinal tissues from 1.3 to 2.2 times as compared to the control (p < 0.05).