The Search for Sources of Enterobacteria and Clostridia Endotoxins in Russian Dairy Farms: Possible Transfer of Endotoxins Through the Feed-Cow-Milk Chain

会议论文
发表日期：

2024年

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Elena Yildirim,
Larisa Ilina,
Georgi Laptev,
Daria Tyurina,
Valentina Filippova,
Andrei Dubrovin,
Natalia Novikova,
Kseniya Kalitkina,
Ogulgerek Djepbarova,
Ekaterina Ponomareva,
Alisa Dubrovina,
Irina Klyuchnikova,
Natalya Patyukova,
Darren Griffin,
Michael Romanov

摘要

Here, samples to identify bacterial endotoxins were collected from two commercial dairy farms in Leningrad Oblast: farm A (samples of feces and milk were taken) and farm (where samples were taken from the feeding table, milk and rumen chyme). The study comprised four groups (A1, A2, B1, B2) where 1 was the control and 2 the test group. A1 were healthy, A2 showed signs of pathologies of the limb joints, B1 received the basic diet (BD) and B2 were fed this, plus the feed additive AntiKlos. Using PCR, samples were examined for the presence of genes for Shiga toxins (stx1A, stx2B), intimin (eae) and enterohemolysin (ehxA) produced by enterobacteria; for alpha (cpa1), beta (cpb) and epsilon toxin (etx) produced by Clostridium perfringens, plus binary toxin (cdtB), toxin A (tcdA) and toxin B (tcdB) produced by Cl. difficile. In the test Group A2, one animal out of six studied (16.7%) had the intimin (eae) and enterohemolysin (ehxA) genes produced by enterobacteria that were not found in the control A1. The epsilon toxin gene (etx) was the most common and present in 100% of the examined fecal samples from both farms and 100% of milk samples from farm A.