Fluorescence Imaging
摘要
In this chapter, fluorescence imaging and microscopy are introduced in detail. It is the fundamental mechanisms, different imaging modalities and complex techniques facing the frontiers of biological and medical research. A comprehensive overview of fluorescence imaging and microscopy is given, including advanced techniques as well. From understanding fundamental mechanisms to exploring cutting-edge methods such as two-photon microscopy and fluorescence lifetime imaging, researchers are equipped with powerful tools to unravel the complexity of biological systems at the molecular level. Fluorescence imaging plays a key role in shaping our understanding of cellular dynamics, with great potential for future discoveries in computational imaging and life sciences.