Texture Features and Machine Learning Based Environmental Microorganism Microscopic Image Classification

In recent years, with the continuous development of molecular biology technology, the effective use of environmental microorganisms to solve the problem of pollution in the ecological environment has been widely used. However, the original microbial cultivation, environmental application and monitoring have the disadvantages of high degree of specialization, long time and high cost. In this case, the use of computer image processing and machine learning techniques for microbial identification and classification becomes a more efficient, economical and necessary method. This paper presents an environmental microorganism microscopic image classification method based on texture features and machine learning. In the research and experiments, microscopic images of microorganisms are cropped, grayed, data augmented, target areas segmented and extracted four types of texture features. The training set and test set are divided in a 1:1 ratio, and different recognition results are obtained based on different texture feature vectors and classifier models. Ultimately, the gradient-gray co-occurrence matrix feature combined with the Random Forest algorithm classifier achieves an F1-measure value of nearly 40%, which improves the work efficiency of environmental microbial researchers and related field workers.