Application of CRISPR–Cas Technology in Drug Development

The discovery of clustered regularly interspaced short palindromic repeat (CRISPR)-Cas technology has transformed the science of genome editing, providing exemplary accuracy and efficiency in changing DNA sequences. It has generated a resurgence in genetic engineering that offers benefits in various sectors, including basic scientific research and translational medicine. By allowing us to alter, discover, and identify certain gene sequences, these genome editing techniques have expanded the possibilities for scientific investigation. The CRISPR–Cas9 system has gained significant acceptance throughout the research community in a very short period due to its extraordinary simplicity, resilience, and versatility. Its uses span various activities, including the construction of transgenic animals and plants, the clarification of disease processes, the discovery of disease targets, the generation of model cell lines, and the control of gene expression. This technology also emphasizes how crucial it is to monitor its development closely. Starting with its invention and emphasizing significant developments in the realm of drug discovery, this review article seeks to offer a thorough overview of the present status of CRISPR–Cas technology in a tailored style, future trends, their opportunities, challenges, and ethical issues that could arise from the continuous advancement and use of this technology.