Changes in the Proportion of Membrane Proteins by Mutation Simulation

We Performed mutation simulations on the whole-genome sequence to determine how random mutations affect the proportion of membrane proteins. We performed two types of mutation simulations: one at the amino acid level with fixed amino acid composition and one at the DNA level with fixed nucleotide composition. In both cases, the distribution of membrane proteins matched the actual values only when the actual composition was fixed. In other words, if the nucleotide composition was the same as the actual genome, the amino acid composition would be the actual value even with random mutations, and moreover, the proportion of membrane proteins would be the same. The results of the mutation simulation naturally led to a mechanism by which random mutations determine the distribution of proteins.