Chapter will look at new cutting-edge methods of corneal regeneration, with a focus on the use of bioscaffoldsBioscaffolds to treat various corneal diseases. Several pathologies can damage the cornea, including infections, hereditary illnesses, degeneration, trauma, and dry eye syndrome. Treatments used, such as corneal transplants and artificial corneasArtificial cornea, have severe drawbacks, including donor shortages and immunological rejection. Study investigates the enormous potential of bioscaffolds, which are designed to replicate the native extracellular matrix and assist cell adhesion, proliferation, and differentiation. In addition, bioscaffoldsBioscaffolds can be employed to transport medications and stem cellsStem cells. The mechanical characteristics and biocompatibilityBiocompatibility of many materials are reviewed, including natural polymers such as collagenCollagenand chitosanChitosan, as well as synthetic polymers. ElectrospinningElectrospinning, 3D bioprinting3D bioprinting, hydrogelHydrogel formulations, and decellularizationDecellularization are among the fabrication techniques being tested for their ability to produce effective bioscaffolds. The chapter also discusses several functionalization options for improving scaffold performance, such as surface modification and the addition of growth agents and nanoparticles. The integration of stem cellsStem cells, particularly limbal, mesenchymal, and induced pluripotent stem cells, with bioscaffoldsBioscaffolds appears to be a potential technique for corneal tissue engineeringTissue Engineering (TE). Despite advances, other difficulties such as immunogenicity, scaffold designScaffold design, and the requirement for specific manufacturing methods must alsoRegenerative medicine be addressed.