The gradual contamination resulting from heavy metals poses a substantial challenge to global endeavors in environmental sustainability. Chromium (Cr) and Arsenic (As) stand out as particularly hazardous to human well-being. The utilization of plants for the removal of such contaminants from the environment, known as phytoremediation, emerges as a promising technique to existing remediation techniques. In the current experimental investigation, the application of atomic absorption spectrometry (AAS) facilitated the assessment through mass balance of Chromium (Cr) and Arsenic (As) levels in both the substrate and various plant species, namely Phragmites karka, Canna indica, Iris, Sagittaria, and Phragmites australis. The findings show mean Cr concentration of 172 mg/kg in the substrate, while the As concentration was measured at 157 mg/kg. Chromium (Cr) and Arsenic (As) exhibited an average highest concentration in the roots and shoots by P. australis (109.9 mg/kg and 91.9 mg/kg for Cr and 131.4 mg/kg and 34.1 mg/kg for As) and P. karka (108 mg/kg and 90.6 mg/kg for Cr and 132.2 mg/kg and 34.3 mg/kg for As), respectively, compared to the substrate and other plants. For both Cr and As, plants removal and tolerance capacities expressed and quantified as bioconcentration factor values (BCF > 1) and translocation factor values (TF < 1) with (p < 0.05). These findings suggest that Phragmites australis and Phragmites karka are a competent species with promising results for the phytoremediation of Cr and As laden wastewater.