The development of flexible and highly sensitive strain sensors has gained significant attention due to their potential applications in various fields such as robotics, healthcare and structural health monitoring. The PVDF nanocomposite nanofibrous samples were prepared through electrospinning with 5wt% ZnO and 10wt% ZnO. The XRD diffraction responses confirm the enhancement of the β phase at 20.2° and diminishing of α phase at 18.5°. The thermal stability and polymorphic polar phase of the PVDF nanofibers were enhanced with addition of ZnO nanofillers due to nucleation and in situ effect. The strain sensor was developed through fabrication with silver paste coated PVDF nanofibrous samples which is tested by the copper electrodes. The voltage output is enhanced for the PVDF/5%ZnO and PVDF/10%ZnO as 1.932 ± 0.05 V and 2.117 ± 0.05 V with 1000 microstrain. The sensitivity of the PVDF/ZnO strain sensors were increased from 0.8953 to 1.6142 V/N. This study contributes to the development of highly sensitive and flexible strain sensors for a wide range of practical applications.