This pilot study evaluated the effects of floor-seated, chair-seated and standing work postures on musculoskeletal discomfort and computer-typing performance. A randomized, repeated measures experimental design was used with 16 participants performing 45-min computer-typing tasks in the three work postures. Musculoskeletal discomfort was measured using a modified Standard Nordic Questionnaire; computer-typing performance was measured by typing-speed and typing-accuracy. Results suggest that musculoskeletal discomfort for whole-body as well as lower-body was significantly lower in the floor-seated work posture compared to the standing work posture. These changes may be attributed to differences in lower-body movement and in weight-distribution for the lower limbs between these two work postures. There was no significant difference in musculoskeletal discomfort between floor-seated and chair-seated work postures. Computer-typing performance between floor-seated, chair-seated and standing work postures did not vary significantly. Considering these findings in totality, the adoption of floor-seated work posture may offer a beneficial alternative to standing work posture for short-duration computer-typing work. Findings from this study should inform the next stage of empirical research investigating interrelationships between work posture, task performance and musculoskeletal discomfort.