Background: Construction workers work under difficult conditions and with inflexible working hours. Quality of life is important in the lives of individual, and to evaluate and analyze the factors affecting construction workers. Objective: This study aims to examine the relationship between depression, stress, social support levels and quality of life in construction workers. Methods: The study included 88 individuals. Quality of life was assessed using the WHOQOL-Brief; for depression level was used Beck Depression Inventory; for social support was used the MSPSS, and stress levels were used the PSS-4. Results: There was a negative correlation between WHOQOL-Brief and Beck Depression Inventory (r = –0.507, p < 0.01) and PSS-4 (r =  –0. 423, p < 0.01), and there was a positive correlation between the MSPSS and WHOQOL-Brief (r = 0.328, p = 0.002). Discussion: In construction workers, increased levels of depression and stress and decreased levels of social support negatively affect their quality of life. Therefore, stress, social support, and depression should be evaluated in detail, and employers should consider these factors to improve the quality of life and well-being of their employees.