Present-day forensic DNA analysis primarily relies on the length-based analysis of STR alleles to identify suspects. This technique poses several limitations, such as limited usefulness in degraded biological samples, mixture interpretation, underutilisation of allelic richness, and many others. In this regard, the next-generation sequencing (NGS) technique helps to identify the correct alleles in terms of their sequence variation, thus increasing the allelic richness in various STR markers. This not only increases the discrimination power but also becomes highly useful in mixture interpretation. The NGS-based approach becomes highly valuable in SNP-based approaches for predicting ancestry, lineage, and phenotypes, thus generating valuable investigative leads. This chapter describes the development of the NGS technique and its advantages in forensic DNA analysis.