Chewing gum was associated with increased productivity and reduced cognitive errors, including reduced stress and anxiety. Increased pressure is one of the common factors contributing to poor golf putting performance. This study investigated the effect of 4 weeks of chewing gum on psychophysiological variables and putting performance among female golfers. Forty-one female golfers participated in a true experimental randomized pre-test and post-test control group study. The golfers were divided into two groups: (1) Chewing Gum Group (CG) (n = 20) and (2) Non Chewing Gum Group (NCG) (n = 21). The CG underwent an intervention of 4 weeks of daily gum chewing, one pallet daily, and chewing for at least 10 min at a time. Whereas, the NCG group was restricted from chewing gum for 4 weeks. The parameters such as anxiety level (CSAI-2 scores), heart rate, and putting scores were taken. Results showed that there was a significant (p = 0.00) improvement in the post-putting score in the Chewing Gum Group (3.00 ± 0.92) compared to the Non Chewing Group (2.10 ± 0.89). A non-significant but positive trend showed that in the Chewing Gum Group anxiety was reduced by 4.11% [Pre; 55.90 ± 8.10 to Post; 53.60 ± 3.84], heart rate was reduced by 3% [Pre; 92.02 ± 6.43 to Post; 89.30 ± 8.68] and an improvement for putting scores in the Chewing Gum Group by 25% compared to Non Chewing Gum Group had a decline in putting performance by − 11.8%. Therefore, the study concluded that chewing gum for 4-weeks may be an inexpensive intervention that improves putting performance and reduces anxiety.