This study examined the physical self-perception (PSP) of university undergraduates and investigated gender and physical activity level differences in PSP. A total of 420 undergraduates (Age: 20.12 ± 1.26 years; male = 62%, female = 38%) from five public universities were surveyed. This study employed a 30-item PSPP questionnaire with 5 subdomains of 6 items each. The International Physical Activity Questionnaire-Short Form was used to assess physical activity participation. Upon receiving institutional research ethics approval and permission of the head of student affairs departments, formal briefings on research objectives, procedures, potential risks, and benefits, emphasizing the voluntary nature of participation were organized at each university. Once agreed, participants signed consent forms and responded to the survey. Total PSP scores revealed that 78–86% of the participants showed strong positive perception (PSP scores > 75) according to gender. Males exhibited a stronger positive perception as compared to females. High positive perceptions were demonstrated by 40.5% undergraduate with high PA levels, and 41.7% moderate PA levels, while lower PSP scores (< 75) was achieved by 17.4% individuals with low PA levels. However, total PSP scores and PA levels were moderately correlated. Conclusion. A high level of PSP increases physical activity participation.