Previous studies have primarily focused on microbubble dispersions using synthetic surfactants, leaving a gap in understanding the behavior of natural non-ionic surfactants, particularly in optimizing foam stability for effective CO2 sequestration. This study investigates microbubble dispersions stabilized with Acacia concinna (Shikakai) surfactant under varying conditions of surfactant concentration, pH, and brine concentration. The Sauter mean bubble diameter, ranging from 61 to 114 μm, and the high gas volume fraction of 81–87% demonstrate the surfactant’s effectiveness in creating highly aerated dispersions, suitable for enhanced oil recovery (EOR) applications. Moderate salt concentrations (1000 ppm NaCl) enhance foam stability, increasing the half-life duration to 160 min due to reduced electrostatic repulsion and improved micelle formation. Conversely, excessive salt concentrations (3000 ppm NaCl) decrease foam stability, with the half-life duration dropping to 104 min, attributed to the salting-out effect and reduced surfactant solubility. Higher surfactant concentrations improve stability across pH values from 5 to 10, with non-ionic surfactants showing less sensitivity to pH changes. This work addresses the complex interactions at the gas–liquid interface, particularly how pH and brine concentration influence foam stability in the presence of CO2. The study provides valuable insights for optimizing surfactant formulations in EOR, enhancing CO2 sequestration efficiency, improving oil recovery rates, and contributing to global efforts to mitigate climate change through sustainable resource management and environmental protection.