This paper reports an Ion-Sensitive Field-Effect Transistor (ISFET) in the precise detection of urological conditions, with a focus on advancing healthcare diagnostics. The sensitivity of the ISFET sensor has been meticulously examined using urine as the electrolyte solution at four distinct temperatures: 273.15 K, 298.15 K, 323.15 K, and 373.15 K and obtained the sensitivities 49, 54, 58, and 67 mV/pH, respectively, revealing an ascending trend of increasing sensitivity with higher temperatures. This trend underscores the profound impact of temperature on ISFET performance, providing crucial insights for tailoring and optimizing sensor deployment in healthcare applications. These research findings may hold substantial promise for the development of precise and efficient diagnostic tools for urological conditions, ultimately contributing to enhanced healthcare outcomes.