Organ regeneration and tissue engineering hold a significant position in life science research. Tissue repair and regeneration are typically accompanied by alterations in the complex microenvironment and endogenous pathways within the organism. In conjunction with engineering techniques, biomaterial-mediated reactions can be employed to expedite tissue repair processes. Among these, intelligent biomaterials responsive to stimuli can achieve the construction of specific cells and microenvironments under human guidance, providing more advantageous conditions for the regeneration of tissues and organs. These external triggers interact with intelligently responsive biomaterials, enabling the biomaterials to act as a bridge between in vivo and in vitro, bridging pathological gaps for electrical signal transmission, biochemical signal changes, drug release, cell loading, and mechanical stress regulation. Future research should focus on the development and application of stimulus-response biomaterials to further optimize biomaterial-based combination strategies. With the deepening of research, more intelligent responsive biomaterials will enter clinical applications and hold great potential in the field of biomedical research.